UTSA | COEHD | University of Texas at San Antonio | College of Edcuation and Human Development
Tue, October 22, 2019

PI: Eunhee Chung, Ph.D.

Lab Members:

Master student- Kassandra Gonzalez

Current Undergraduate Students: Antonio Bollinger, Jacqueline Gonzalez, Erin Roye, Christina Perez, and Kimberli Garcia.

Lab Alumni: Justin Cruger (M.S. graduated in August 2017), Salvatore Campise (B.S. graduated in December 2017) and Jake Sansom (B.S. graduated in December 2018). Andrea Chinas Melvin (summer research student from Monterrey Institute of Technology, Mexico).

Research Interests:

My research has focused on two agendas: First, we investigate the impact of maternal diet and exercise on the cardiometabolic health of offspring. We wish to probe the mechanisms by which maternal exercise before and during pregnancy is a modifiable maternal behavior that may reduce the development of obesity-linked cardiometabolic dysfunction in offspring. Second, we have studied diet-induced obese mouse models to better understand the role of nutritional supplementation in type 2 diabetes, particularly skeletal muscle adaptation. An interdisciplinary approach, including functional measurement (echocardiography, pressure-volume hemodynamics, and myocyte contractile properties), mitochondrial function, gene expression, biochemical assays, immunohistochemistry, and histology has been used to investigate these topics.

Publications:

https://www.ncbi.nlm.nih.gov/sites/myncbi/18Og94g7_mpQd/bibliography/51981910/public/?sort=date&direction=ascending

Major Laboratory Equipment:

Echocardiography- in vivo cardiac function measurement

Echocardiographic measurement is performed under light anesthesia to measure cardiac function

Analysis of Echocardiography


O2K-FluRespirometry: Mitochondrial functional measurement

Mechanical preparation of cardiac fibers to measure mitochondrial function. Cardiac tissue is separated into small bundles ( 1mm diameter and about 2 mm length strip) to prepare saponin-skinned fibers

Mitochondrial function measurement using O2k-FluoRespirometry


Real-time quantitative PCR machine to measure gene expression


Chemiluminescence detection system to obtain an image

After running the gel electrophoresis, the gel was silver-stained, and then image was analyzed to quantify the skeletal muscle fiber composition (Type I, IIa, IIx, and IIb)


Cryostat and A fluorescent microscope: Immunofluorescence Analysis

Frozen muscle sections are cut into 8mm thick cryosection to characterize the skeletal muscle



Antibody treatment to the cryosection - primary antibodies were added to the muscle section to visualize the muscle fiber 



Different colors indicate different muscle fiber (Blue- Type I, Green- Type IIa, Red- Type IIb, Unstained- Type IIx)


Kinesiology, Health, and Nutrition




Upcoming Events



Doctoral Info Session: Educational Leadership

10-23-2019

TIME: 6:00-7:00pm

LOC: DT Campus

BLDG: Durango Building, El Paseo Room B 1.120


Alamo STEM Ecosystem Professional Development Conference Proposals

Proposal Due: October 25, 2019

TIME:

LOC: Other

BLDG:


Fall 2019 Graduate Studies Open House

10-26-19

TIME: 9:30am

LOC: DT Campus

BLDG: Frio Street Building, Ground Floor Commons


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